The Laboratório Associado Institute for Biotechnology and Bioengineering (IBB) is a research and development (R&D) unit, founded in October 2006 aiming to be a strategic infrastructure for the development of the Portuguese R&D and innovation policies in the areas of Biotechnology, Bioengineering, Biomaterials and Life, Biomedical and Agricultural Sciences. IBB combines its R&D activities with advanced education, technology transfer, consulting and services, with the aim of fostering the industrial, health, agriculture and environmental sectors.
The Laboratorio Associado Institute for Biotechnology and Bioengineering (IBB) is a research and development (R&D) unit, founded in October 2006 aiming to be a strategic infrastructure for the development of the Portuguese R&D and innovation policies in the areas of Biotechnology, Bioengineering, Biomaterials and Life, Biomedical and Agricultural Sciences. IBB combines its R&D activities with advanced education, technology transfer, consulting and services, with the aim of fostering the industrial, health, agriculture and environmental sectors.
IBB is a nation-wide Institution and a partnership of five research units funded by “Fundacao para a Ciencia e a Tecnologia”, from Ministry of Science, Technology and Higher Education, Portugal. These centres, which are based in university campuses located in the North (Braga and Vila Real), Centre (Lisboa) and South (Faro) of Portugal, are:
IBB has a strategic and ambitious research plan to respond to the new challenges resulting from the extraordinary developments and breakthroughs in subjects such as Molecular and Cell Biology, Genomics, Systems Biology, Biomaterials, Cell and Tissue Engineering, and Nanotechnology.
The IBB contribution to the development of these cutting-edge and fast growing/moving topics relies on the integration of different scientific and technological subjects and competences of excellence, through six interdisciplinary Research Thrust Areas:
1. Biomolecular Science and Engineering
2. Genetics, Molecular and Cell Biology
3. Functional, Comparative and Evolution Genomics
4. Bioprocess and Biosystems Engineering
5. Biomaterials for Tissue Engineering and Regenerative Medicine
6. Catalysis and Reaction Engineering
These research areas support the IBB activities focused on four Thematic Areas:
IBB activities are focused in four thematic areas:
Industrial biotechnology concerns the application of modern biotechnology for the industrial production of added-value chemical substances and bio-energy, using inherently clean processes, with waste minimisation and reduced energy consumption. Multidisciplinary activities are pursuit in which biochemistry, microbiology, molecular and cell biology, genomics and bioinformatics and process engineering are integrated and combined aiming the development of useful processes and products based on microbial, animal or plant cells, their organelles or enzymes as biocatalysts.
The Laboratorio Associado IBB is committed to develop R&D activities within the thematic area of Industrial Biotechnology on three major topics:
A) Biological and Chemical Processes – Molecular and Cell Engineering; Reaction Engineering; Purification of Biomolecules and Valorisation of Natural Products; and Process design.
B) Nanobiotechnology – Micro- and Nano-devices; Nano-structured catalytic materials; and Nanoparticles
C) Modelling of Biological and Chemical Systems - Computational and predictive models; and Biosystems analysis
Within the thematic area of Health Biotechnology, the Laboratorio Associado IBB is committed to develop R&D activities specifically on four major aspects:
A) Molecular Determinants and Mechanisms of Disease – Drug resistance and Microbial pathogenesis and epidemiology; Polymorphism screening and cancer susceptibility; Cancer molecular cyto/genetics; Protein function, structure, activity and their implication in disease onset; and Genetic control of organogenesis.
B) Diagnostic Tools - Generic biochip and biosensors platforms; and Comparative genomics, transcriptomics and proteomics techniques for gene expression identification and diagnostics.
C) Molecular Therapeutic Technologies -Vectors for gene therapy and vaccination; and Controlled/sustained drug delivery and targeting.
D) Stem Cells, Biomaterials and Tissue Engineering – Stem Cell and Tissue Engineering; Novel biomaterials and biomaterials modification, bioactivity and biomimetic coatings.
The thematic area of Agriculture Biotechnology focuses on the application of expertises in functional, comparative and evolution genomics, molecular genetics, to biological systems of interest in agriculture, animal biotechnology and food processing.
The Laboratorio Associado IBB is committed to develop R&D activities within the thematic area of Agricultural Biotechnology on five major topics:
A) Genetic Variability - Natural and induced (mutagenesis) genetic variability in plants, animals and microorganisms.
B) Plant and Animal Functional, Comparative and Evolution Genomics - Comparative and evolution genomics of crop and forestry species and mammalian species; Molecular cytogenetics; and Genome organisation in plants and animals.
C) Genetic Resources and Plant breeding - Genetic resources preservation strategies and plant breeding
D) Plant and Animal Biotechnology - In vitro culture and micropropagation; Selection of DNA markers in animals and plants; Proteomics of metal ions stress in plants; Somatropic genes variants and alternative transcripts in sheep; and Food traceability.
E) Microbiology and Wine Technology - Identification, physiology, metabolism and genomic, transcriptomic and metabolomic characterisation of wine microorganisms.
ENVIRONMENTAL BIOTECHNOLOGY AND CHEMISTRY
The main objective of this area is to respond to industrial and society demand for efficient environmental protection technologies through the combination of process development and fundamental research on the biological and chemical phenomena supporting the currently implemented industrial processes. The development of innovative technologies for industrial pollution prevention, control and monitoring, and for industrial wastewater treatment, and identification of the biological effects of exposure to polluting agents are pursuit.
The Laboratorio Associado IBB is committed to develop R&D activities within the thematic area of Environmental Biotechnology and Chemistry on four major topics:
A) Integrated Pollution Prevention and Control - Integrated approaches for waste and/or effluent minimisation; and Implementation of expert systems.
B) Treatment Processes and Technologies – Analysis of natural and artificial ecosystems; and Novel treatment technologies of highly toxic substances.
C) Bio/catalytic Materials and Processes for Gas-phase Effluent Treatment – VOC reduction; and NOx and SOx elimination.
D) Non-Conventional Biological Diagnostic and Monitoring Systems – Image analysis; Biological models; and Environmental biosensors.
RESEARCH THRUST AREAS
The members of IBB have developed expertises and competences in six thrust areas:
Biomolecular Science and Engineering
Protein and Enzyme Engineering is focused on the design of functionally active and stable proteins, namely enzymes and therapeutic proteins, using in silico structure analysis and modelling, recombinant DNA technology, directed evolution, protein immobilisation, spectroscopic techniques and medium engineering. The assessment of structural stability, folding/unfolding pathways and protein-protein interactions can provide insight on the mechanisms underlying the formation of protein deposits occurring in amyloid and prion diseases. Mechanisms of protein assembly are also essential to build protein complexes such as the proteasome, chaperones, oligomeric enzymes, and virus-like nanoparticles.
Nucleic Acid Engineering is focused on DNA-based technology for gene therapy, diagnostics and vaccination. Particular attention is given to the design of plasmid vectors and selected hosts as well as to structural and stability studies of plasmid DNA to facilitate purification and enhance biological activity.
Nanobiotechnology, integrates nano/microfabrication and biosystems and generates powerful methods that allow substantial new insights into biological systems function. Microelectronically based chips are developed to design and assemble devices for genetic analysis. Screening and identification of single nucleotide polymorphisms (SNPs) and gene expression patterns are performed by hybridising appropriate biological samples on microarray devices (DNA chips). Charge transfer through DNA is used for the direct transduction of DNA hybridisation on gold microelectrodes and for the simultaneous detection of DNA polymorphisms by electrochemical methods. Piezoelectric and optical transduction based on energy transfer and fluorescent quenching using semiconductor nanocrystals are also investigated. The effects of nano (and micro) patterning on cell adhesion and differentiation are also assessed.
Genetics, Molecular and Cell Biology
The Genetics, Molecular and Cell Biology thrust area aims at developing research programmes in the domains of Life, Biomedical and Agricultural Sciences at the cellular and molecular levels.
IBB research activities focus on the molecular mechanisms of response of cells to chemicals (drug/xenobiotic/endobiotic) or other stress stimuli. In this context, yeast cells, human cell lines, primary animal cell cultures, knocked-out mice and other animal models, are used to identify and elucidate the role of multidrug resistance (MDR) and ABC transporters, as well as drug metabolising enzymes (DME) in the cell response to stress and in the mechanism of acquired resistance.
Pharmacogenetics, gene expression approaches, DNA pooling strategies, and multiplex amplification and genotyping methodologies, are used to evaluate the effect of inter-individual genomic variability in determining the individual outcome of therapeutics as well as in epidemiological studies. Of particular interest is the evaluation of the association and role of polymorphic genes involved in the metabolic fate of xenobiotics, DNA repair, and in the control of cell cycle in oncological, cardiovascular, and infectious diseases. Large epidemiological surveys are also realized in different disease settings in particular focusing bacteria belonging to the Burkholdera cepacia complex (Bcc) and human DMEs in cystic fibrosis and malaria infection, respectively.
Polymorphism analysis is also used to study genetic variability in plants, animals and microorganisms. Particular focus is given to crop species, fruit trees and forestry species, in bovidae and suinae species; and to the identification and strain differentiation of Lactic Acid Bacteria and Saccharomyces cerevisiae in fermentation and wine production.
Functional, Comparative and Evolution Genomics
The Functional, Comparative and Evolution Genomics thrust area aims at developing integrated and cross disciplinary research programmes in the domain of Life, Biomedical and Agricultural Sciences by applying the experimental and computational approaches of the post-genomic era.
Combined approaches taking full advantage of the potentialities of Saccharomyces cerevisiae, chick embryos, mouse transgenics, embryonic stem cells, and other cellular or animal models, of gene targeting, identification, manipulation, and analysis technologies (i.e. siRNA and GeneChip technologies), and of bioinformatic tools are used to investigate signaling pathways, gene regulation, expression, and function and transcriptome analysis. Such approaches are used in topics of biomedical relevance such as brain development and vertebrate hemangioblast/cardiogenesis, and to elucidate the regulatory networks underlying yeast responses to a number of chemical stresses of interest in Food and Biotechnological Industries, in Agriculture, and in Medicine. Similarly, systems biology approaches, involving the combination of in silico, comparativefunctional genomics and proteomics, are applied to other microorganisms, mainly Pseudomonas related bacteria and pathogenic yeast species Candida albicans and Candida glabrata.
Comparative Genomics of forest and crop species is performed using heterologous probes such as genomic DNA from wild relatives, repetitive DNA sequences and transgenes in order to evaluate the rearrangements of the genomes of closely related species and infer the results under an evolution point of view. Molecular Cytogenetic approaches are applied to analyse pre-meiotic and meiotic events in developing wheat, rye and barley anthers, and to investigate the organisation of transgenes delivered into wheat, tritordeum, Vitis and other genomes.
Bioprocess and Biosystems Engineering
Bioprocess engineering is focused on the development of processes, based on microbial and animal cells, to produce engineered biomolecules and valuable metabolites. Bioconversion processes in conventional and non-conventional media are carried out in selected type of bioreactors. Bioreactor characterisation and design include microbioreactors, multiphase bioreactors, biofilm reactors, oscillatory flow reactors, hyperbaric bioreactors and cell culture reactors for the production of cells and bioactive molecules, as well as for food and environmental applications.
Process development for production and purification of antibodies, growth factors, proteins, nucleic acids, and nanoparticles and nano-structures for drug delivery are performed, matching the growing industrial need for biopharmaceutical and nanobiotechnology applications. Novel bioseparation methods are established aiming high product purity, process consistency and economy.
Stem Cell Bioengineering research is focused on the development of bioreactor culture systems for human hematopoietic stem/progenitor cells (HSC), human mesenchymal stem cells (MSC) and mouse embryonic stem cells (ESC) in an ex-vivo environment. The molecular and cellular mechanisms involved in expansion and differentiation, the kinetics of the ex-vivo stem cell expansion/maintenance and differentiation are addressed. Predictive kinetic models are used to describe stem cell fate. Priority is also given to novel concepts and devices for stem cell isolation and differentiation.
Biosystems Engineering explores the development of Systems Engineering to bioprocess monitoring and control and the integrative mathematic and chemometric approaches for elucidation and analysis of biosystems. Advanced process monitoring and control and novel sensor technology, such as intelligent real-time control systems with on-line learning capabilities, are studied within the framework of process analytical technologies (PAT) applied to biological and pharmaceutical engineering production.
Biomaterials for Tissue Engineering and Regenerative Medicine
Biomaterial surface modification is performed to manipulate and optimize key surface parameters for cell adhesion and proliferation as well as for biomolecule immobilisation in different tissue engineering applications, micro and nano biological sensors, and enzyme immobilisation.
Functional tissue engineering hybrid constructs, combining human mesenchymal stem cells (MSC) technology and materials science are developed aiming at bone and cartilage replacement/regeneration. Biomaterial scaffolds are seeded in vitro with human microvascular endothelial cells to evaluate endothelial cell attachment and anginogenesis.
Catalysis and Reaction Engineering
Molecular and kinetic modelling techniques are applied to understand and improve existing chemical processes as well as to complex systems, namely biological systems, such as enzyme catalysis and animal cell expansion and differentiation.
Catalysts, either zeolite or non-zeolite based, are developed for industrial applications, for catalytic transformation of hydrocarbons for the petroleum and petrochemical industries. Nano-structured materials are also designed aiming environmental applications in particular for pollutant reduction including VOC and NOx elimination.
The bodies of Laboratório Associado IBB are:
The Board of Directors
directors, or their representatives, of the Research Units, IBQF, CEB-UM, 3B’s-UM, CGB-UTAD and CBME-UAlg. The director of IBQF is the Director of the Laboratório Associado IBB, who chairs the Board of Directors and is responsible for the overall management.
The Board of Directors members are:
The Executive Office
The Executive Office assists the Director in the overall management of the Laboratório Associado IBB resources. It is chaired by the Director and integrates IBB Faculty Members, the Liaison Officer and a secretariat.
The Scientific Coordination Committee
The Scientific Coordination Committee is composed by the Director of IBB, the Leaders of the Thematic Areas and of the Research Thrust Areas. The Director of IBB chairs this committee.
The Scientific Coordination Committee members are:
The Scientific Council
This council integrates all Doctorate members of IBB.
The International Scientific Advisory Committee
Composed by top scientists from Europe, North America and Japan/Asia.
The mission of the Institute for Biotechnology and Bioengineering (IBB) is to promote excellent quality research and development, advanced education and technology transfer, ensuring national and international competitiveness in the Biotechnology, Bioengineering, Biomaterials and Genetics domains for the benefit of the industrial, health, agricultural and environmental sectors. The specific goals are to:
1. Conduct excellent quality and competitive research in the domains of activity of IBB by establishing effective collaborations between scientists and engineers.
In order to fulfil its mission and attain the goals, IBB undertakes essentially four types of activities:
Scientific and technological research through: (i) fundamental research to expand the boundaries of current knowledge and to ensure the development of innovative biological and chemical processes; and (ii) applied research to exploit such knowledge through the development and implementation of integrated, biological, chemical and physical processes, which are competitive, innovative, cost effective, reliable, robust, safe and well controlled, thus improving process efficiency, economy and sustainability.
Advanced education and training of a vast audience (undergraduate and postgraduate students, professionals). The IBB research units are responsible for the majority of the national Graduation courses in Biotechnology and Bioengineering. IBB participates in the PhD Programme “Bioengineering Systems” within the agreement of the Portuguese Government and the Massachusetts Institute of Technology, USA. IBB will pursuit and foster post-graduation programmes, at the Master and PhD level (2nd and 3rd cycles) in the different domains of Biotechnology and Bioengineering.
Services, consulting and technology transfer for the productive sector not only for the restructuring of the traditional chemical and biological industries (pharmaceutical, chemical, food, etc.), but also as a way of creating new opportunities in the industry and agriculture. IBB incentives the patenting, licensing, outsourcing and implementation of R&D projects in cooperation with established companies, start-ups or spin-offs. Consulting, contract research and other services (e.g. advanced analytical techniques) are made available to the biological, chemical, instrumentation and diagnosis industries.
Dissemination, scientific awareness and public perception through publication in International scientific journals and conferences and organisation of cutting-edge conferences, workshops, short courses, etc., on IBB main areas of expertise. The opportunities and benefits arising from the use of biological, chemical and biomaterials technology are also addressed and balanced against concerns over potential socio-economic and physical risks to human health and the environment.
Instituto de Biotecnologia e Bioengenharia
Av. Rovisco Pais
1049-001 Lisboa – Portugal
Phone: +351 21 841 9065
Fax: +351 21 841 9062